ZFIN Image: Nowak-Sliwinska et al., 2018, Fig. 7

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Fig. 7

Serum-free defined model of human endothelial cell-pericyte tube co-assembly in 3D collagen matrices. a Human EC and GFP-labeled pericytes (Peri) were seeded together at the indicated cell densities and after 120 h, were fixed and stained with anti-CD31 antibodies. The immunostained cultures were imaged using confocal microscopy. The serum-free defined culture system contains SCF, IL-3, SDF-1α, FGF-2 and insulin (a component of the RS II supplement), which are required to be added in combination. b Cultures fixed at 120 h were immunostained with antibodies to CD31, laminin (LM), and fibronectin (FN) and were imaged using confocal microscopy or were examined by transmission electron microscopy. Arrows indicate the capillary basement membrane. L indicates lumen while Nuc indicates nuclear labeling. Bar equals 25 μm

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