Fig. 19

Fig. 19

Experimental flowchart of the image-guided laser-induced CNV model and data collection. a Overview of the procedure for CNV induction involving mouse preparation and followed by experimental treatment, sample preparation, and analysis. b Representative image of normal fundus (Green check mark). c Representative image of anomalous structure (white arrow) in the eye, which is not suitable for laser photocoagulation (Red X). d Representative image of normal fundus with four laser burns shown as bright white spots. e Representative image of a successful laser burn (white arrow) with 3D optical coherence tomography. f, g Representative ocular fundus fluorescein angiography images at 5 and 10 min after the injection of fluorescent dye at day 6 after laser burn. h Representative images of flat-mounted choroid with IB4 staining at day 7 after laser photocoagulation. Scale bar: 200 μm. ON: optic nerve. (i) Higher magnification of the laser-induced CNV lesion highlighted in panel H. Scale bar: 50 μm. Reproduced with permission from [467]