Fig. 17

Fig. 17

Identification of tip cells. The tip cell is the leading cell of an angiogenic sprout with long filopodia extensions, followed by stalk cells that proliferate and phalanx cells that form a matured new capillary. a Tip cells in the developing mouse retina can be identified by staining with isolectin B4 (IB4). b The mouse retinal vasculature (IB4) follows the astrocytic meshwork (GFAP) when forming the superficial vascular plexus during development. c A combination of collagen type IV IHC (in pink) for general staining of blood vessels and ISH for PDGFB (black) for specific identification of tip cells in the developing mouse retina. d Tip cells in endothelial cell cultures are identified by CD34