Fig. 8

Fig. 8

EC co-culture spheroid assay. a Overview of the procedure of the hanging drop spheroid growth assay over time. Prior to initiating the co-culture, tumor cells (green) are allowed to establish initial micro-spheroids by themselves. Typically, after 72 h EC can be introduced (red) and their incorporation monitored over time up to 14 days. b After 7 days, the tumor-EC spheroid can be quantitatively and qualitatively evaluated by various techniques, such as c (fluorescence) microscopy, histology, functional assays or implanted in vivo. Breast tumor cells (green; GFP-4T1) are combined with 2H11 EC (red) stained with MitoTracker Red. Scale bar = 100 μm