ZFIN Image: De La Garza et al., 2017, Figure 2

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Figure Caption

Figure 2

Diverse signals regulate embryonic HSC behaviors in zebrafish. (A): Inflammatory signals from myeloid effector cells promote HSC emergence through activation of Notch and NFκB signaling. Tet2/3 also regulate Notch signaling, leading to gata2b and runx1 expression in hemogenic endothelial cells. Cbfβ is subsequently required to promote the extravasation of emerging HSC out of the dorsal aorta. (B): Nascent HSCs seeding the CHT induce endothelial remodeling to form a “microniche” comprising an HSC surrounded by endothelial cells adjacent to a CXCL12‐expressing supportive stromal cell. The orientation of the division plane of the HSC is dictated by the position of the stromal cell. An open arrow on the HSC (red) and daughter cell (green) shows the angle of the division plane. Arrows within the vessels show the direction of blood flow. Abbreviations: AGM, aorta‐gonad‐mesonephros; CHT, caudal hematopoietic tissue; EC, endothelial cell; hpf, hours postfertilization; HSC, hematopoietic stem cell; Ifnα/γ, interferon α/γ; Tnfα, tumor necrosis factor α.

Acknowledgments

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