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Figure 2
Fetal-type splice isoforms are reduced after overexpression of scaRNAs. Data are fold change of exons that represents regions of variability within the alternatively spliced gene. Each value is derived from the mean intensity level from three different primary myocyte cell lines (3 biological replicates with 3 technical replicates for each individual gene analyzed by qRT-PCR). The normal comparison cells (blue) provide a reference point for comparisons between the sham transfected TOF cells and those with the expression vectors. * significantly different from levels in sham transfected TOF primary cells. The TOF primary cells were derived from three different infants and each PCR included 3 technical replicates. Expression plasmids pCGL-ACA26 and pCGL-ACA35 were a generous gift from Dr. Tamas Kiss, Universite Paul Sabatier, Toulouse, France.