Figure 8.

Figure 8.

Inhibition of Wnt-signalling affects morphogenesis. In situ hybridizations for bambia (a–f), vax2 (g–l) and pax2a (m–r) on porcupine inhibitor (LGK-974)-treated embryos (d–f, j–l, p–r) and DMSO control embryos (a–c, g–i, m–o) at 24 hpf. Inhibitor treatment started at 13 hpf. Wnt-signalling inhibition is not affecting the expression domain of bambia but reducing expression intensity. Wnt-signalling inhibition results in ectopic expression of vax2, which can be found in the lens-averted layer of the optic cup. Wnt-signalling inhibition also results in a reduced pax2a expression domain within the optic cup, while the pax2a expression domain in the optic stalk is enlarged and extending into the nasal lens-averted domain. Lateral view, nasal to the left; scale bar, 25 µm. For each condition, eight embryos were used; four to five of them were imaged.