Figure 6

Figure 6

CAVEOLINs Are Negative Regulators of YAP/TAZ In Vivo

(A) Images of epithelial cells from WT and cav1^−/−/cav3^−/− 48 hpf zebrafish embryos labeled for Hoechst (blue) and YAP (red). Note the increased nuclear YAP localization in cav1^−/−/cav3^−/− cells. Scale bars represent 25 μm.

(B) Quantification of cellular nuclear-to-cytoplasmic ratio from cav1^−/−/cav3^−/− superficial epidermal cells compared to WT. Each dot represents one cell. n > 100. Means ± SEM.

(C) Images from cav1^−/−/cav3^−/− and WT zebrafish embryos expressing epidermal EGFP-yap S54A (Tead-binding-deficient zebrafish yap mutant) and H2A-mCherry (nuclear marker). Note the predominantly nuclear YAP localization in the cav1^−/−/cav3^−/− epidermal cells compared to WT. Scale bars represent 20 μm.

(D) Quantification of nuclear-to-cytoplasmic ratio from cav1^−/−/cav3^−/− epidermal cells compared to WT. Each dot represents one cell. n > 50 from each genotype. Means ± SEM (related to Figure S5H).

(E) qPCR analysis from four-day-old cav1^−/−/cav3^−/− and WT zebrafish embryos. Means ± SD.