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Figure 6—figure supplement 1.

ID: ZDB-IMAGE-190723-1080
Source: Figures for Gálvez-Santisteban et al., 2019

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Figure Caption

Figure 6—figure supplement 1. Inhibiting BMP or Erbb2 signaling impairs reactivation of early cardiogenesis transcription factors.

Whole-mount in situ hybridizations show increased (B) gata4 (n = 19/25), (J) hand2 (n = 10/12) and (R) nkx2.5 (n = 12/13) expression in vmhc:mCherry-NTR ventricle-ablated hearts at 48 hpt (7 dpf) when compared to (A, I, Q) sham-ablated control hearts. However, these cardiogenesis transcription factors fail to increase in (D, L, T) dorsomorphin (DM) (n = 4/21 gata4; n = 3/12 hand2; n = 4/16 nkx2.5) and (F, N, V) AG1478-treated ventricle-ablated hearts (n = 5/20 gata4; n = 1/8 hand2; n = 3/10 nkx2.5) as well as in (H, P, X) erbb2 loss-of-function mutation (erbb2^-/-) (n = 4/18 gata4; n = 5/24 hand2; n = 3/15 nkx2.5). V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment. Dashed lines outline the heart.

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