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Figure 5—figure supplement 2.

ID: ZDB-IMAGE-190723-1078
Source: Figures for Gálvez-Santisteban et al., 2019

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Figure Caption

Figure 5—figure supplement 2. <italic>klf2a</italic> and <italic>trpv4</italic> mutants show impaired post-injury re-activation of cardiogenesis transcription factors.

(A–R) Whole-mount in situ hybridizations show increased (B) gata4 (n = 21/24), (H) hand2 (n = 13/15) and (N) nkx2.5 (n = 12/13) expression in vmhc:mCherry-NTR ventricle-ablated wild-type (wt) hearts at 48 hpt (7 dpf) when compared to (A, G, M) sham-ablated control hearts. However, these cardiogenesis transcription factors fail to increase in (D, J, P) klf2a^-/- (n = 7/21 gata4; n = 4/9 hand2; n = 5/14 nkx2.5) and (F, L, R) trpv4^-/- (n = 7/17 gata4; n = 5/11 hand2; n = 5/12 nkx2.5) ventricle-ablated hearts. V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment. Dashed lines outline the heart. Bars: 50 μm.

Acknowledgments

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