|
Fig. 2
LSN 2105786 upregulates Wnt Signaling and produces eyeless phenotype. (A–I) Embryos treated with LSN 2105786 displayed the absent or small eye phenotype in a dose dependent manner characteristic of over-activated Wnt signaling at 28 hpf. (J) Live image of DMSO treated embryo showing normal eye at 28 hpf. (K–S) Embryos treated with LSN 2105786 displayed the absent or small eye phenotype in a dose dependent manner characteristic of over-activated Wnt signaling at 48 hpf. Panels E’, F’, G’, N’ are second examples of each concentration. (T) Live image of DMSO treated embryo showing normal eye at 48 hpf. (U–X) Bright field (U,W) and green fluorescence (V,X) images of the midbrain-hindbrain boundary region of Tg(TOP:GFP) embryos treated with DMSO (U,V) or 450 nM LSN 2105786 (W,X) at 24 hpf. (Y) Graph showing the GFP expressing midbrain-hindbrain boundary region of the embryos treated with different concentrations of LSN 2105786. Compared to DMSO, increased GFP volume in 300–450 nM was statistically significant (p < 0.001), and 500 nM effect was not statistically significant.