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Fig. S11
bif1 knockdown is rescued by co-injection with bif1 mRNA. A. Schematic representation of the genomic structure of bif1. The red line corresponds to the morpholino-targeted site. Dark and light blue arrows correspond to the primers amplifying a long (972 nucleotides) and a short product (224 nucleotides), respectively. The size of the intron is 1122 nucleotides. B. PCR on cDNA obtained from embryos at 24hpf, injected with MO-control or MO-bif1, or non-injected. In bif1-morphants, the wild-type band disappears, suggesting retention of the intron. C. Bright-field pictures of embryos at 3dpf stained with O-dianisidine. D. Quantification of embryos exhibiting the corresponding morphologies, dorsalization and ventralization (smaller eyes) at 3dpf. The numbers of embryos are indicated in parentheses. E. Quantification of embryos after O-dianisidine staining at 3dpf.