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Fig. 4
Details of islet1-GFP expression using fluorescent DAPI stain and choline acetyltransferase (ChAT) immunohistochemistry. Conventions with lettering as in Figure 2. (A,A’) Cholinergic cells in anterior parvocellular preoptic nucleus (PPa) are never double-labeled with islet1-GFP. (B,B’)Cholinergic cells in magnocellular and posterior parvocellular preoptic nuclei (PM, PPp) and suprachiasmatic nucleus (SC) are never double-labeled with islet1-GFP. (C,C’) Shows islet1-GFP positive axonal tract from the ventral nucleus of the ventral telencephalon (Vv) to the medial zone of the dorsal telencephalon (Dm). (D) Magnification shows how hypothalamic tract leads into the medial part of the secondary gustatory nucleus with some collaterals into the central gray. (E,E’) Magnification of midbrain tegmentum shows lateral to the oculomotor nerve nucleus (NIII) the additional cholinergic cells of the nucleus of Edinger–Westphal (NEW; yellow arrows) to be double-labeled for islet1-GFP. Note also the few non-cholinergic cells of the rostral perilemniscal nucleus stained for islet1-GFP. (F,F’)Highlights the few cholinergic/islet1-GFP positive cells (yellow arrows) ventrolateral to the caudal perilemniscal nucleus which itself is islet1-GFP negative, but ChAT positive. #: indicates a ChAT positive fiber net (See Mueller et al., 2004). (G,G’) islet1-GFP cells in the rostral perilemniscal nucleus (PLr). (H–H”) islet1-GFP positive cells in the nucleus of the medial longitudinal fascicle (Nmlf) and cholinergic cells in the dorsal thalamus (DT) and near the preglomerular region (PG). (I,I’) islet1-GFP cells in the Nmlf or possibly the mesencephalic sensory trigeminal nucleus at the level of an islet1-GFP axonal tract that projects from the intermediate hypothalamic nucleus (IN) to the dorsal thalamus. dIV, decussation of trochlear nerve; Dm, medial zone of dorsal telencephalon; DT, dorsal thalamus; DTN, dorsal tegmental nucleus; GC, griseum centrale; Hd, dorsal zone of periventricular hypothalamus; IN, intermediate hypothalamic nucleus; IIf, lateral forebrain bundle; NIII, oculomotor nerve nucleus; NEW, nucleus of Edinger–Westphal; NI, nucleus isthmi; NLL, nucleus of the lateral lemniscus (of Prasada Rao et al., 1987); Nmlf, nucleus of the medial longitudinal fascicle; ot, optic tract; PG, preglomerular complex; PGZ, periventricular layer of optic tectum; PLc/PLr, caudal/rostral perilemniscal nucleus; PM, magnocellular preoptic nucleus; PPa/PPp, anterior/posterior parvocellular preoptic nucleus; SC, suprachiasmatic nucleus; SGN, secondary gustatory nucleus; SRN, superior reticular nucleus; TS, torus semicircularis; Va, valvula cerebelli; Vc/Vd/Vv, central/dorsal/ventral nucleus of ventral telencephalon; IV, trochlear nerve.