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Fig. 3
Details of islet1-GFP expression using fluorescent DAPI stain and tyrosine hydroxylase (TH) immunohistochemistry. Conventions with lettering as in Figure 1. (A,A’) islet1 expression in precommissural subpallium shows positivity in ventral, and negativity in central (Vc) and lateral nuclei (Vl). (B,B’) islet1-GFP expression in supracommissural nucleus (Vs). (C–C”’) islet1-GFP fibers in intermediate nucleus of the ventral telencephalon. Note also TH cells in the anterior parvocellular preoptic nucleus (PPa). (D–D”) Dopamine cells in magnocellular (PM), posterior parvocellular (PPp), and suprachiasmatic nuclei (SC), with only cells in PPp double-labeled for islet1-GFP. (E) Origin of the dorsal accessory optic nucleus (DAO) as suggested by a chain of islet1-GFP cells (arrowheads) that apparently migrate pially from the parvocellular periventricular posterior tubercular nucleus (TPp-p) and merge into the DAO. Straight stippled line indicates midline. (E’) Slightly more medially taken picture shows the parvocellular (TPp-p) and pear-shaped (TPp-m) parts of TPp on both brain sides in TH immunostaining. Note that some cells in TPp-p are double-labeled. (F–G) Analysis of anterior part of caudal zone of periventricular hypothalamus (Hc) and posterior tuberal nucleus (PTN) at rostral (F: DAPI, F’: islet1-GFP, F”: TH stain) and caudal levels (G: DAPI, G’: islet1-GFP, G”: TH stain). Note that there are no TH and islet1-GF double-labeled cells in PTN. ac, anterior commissure; DAO, dorsal accessory optic nucleus; DIL, diffuse nucleus of the inferior lobe; Dl, lateral zone of dorsal telencephalon; Dm, medial zone of the dorsal telencephalon; DP, dorsal posterior thalamic nucleus; E, epiphysis (pineal); ENd, dorsal entopeduncular nucleus; Hc/Hd, caudal/dorsal zone of periventricular hypothalamus; mfb, medial forebrain bundle; PG, preglomerular complex; PGZ, periventricular gray zone of optic tectum; Pit, pituitary; PM, magnocellular preoptic nucleus; PPa/PPp, anterior/posterior parvocellular preoptic nucleus; PTh, prethalamus; PTN, posterior tuberal nucleus; TLa, torus lateralis; TPp-m/TPP-p, magnocellular (pear-shaped)/parvocellular cell part of periventricular posterior tubercular nucleus; Vc/Vd/Vi/Vl/Vp/Vs/Vv, central/dorsal/intermediate/lateral/posterior/ supracommissural/ventral nucleus of ventral telencephalon; SC, suprachiasmatic nucleus; SD, subpallial dopamine cells; VI, abducens nerve.