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Fig. 6
CDC25 inhibitors block the growth of K-Ras-dependent intestinal organoids. (A) Organoids reproducing duodenal crypts and derived from Apc/K-Ras mouse small intestine (left) were grown in the presence of UPD-176 (right). Phase contrast stills were obtained at 48 h of treatment. (B) Confocal immunofluorescence microscopy of Apc/K-Ras organoids treated as in A and stained for proliferation (β-catenin) or differentiation (lysozyme) markers. DNA was stained with DAPI. (C) qRT-PCR on cDNA obtained from control- and UPD-176-treated organoids was conducted using primers to the indicated markers.
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