|
Fig. 6
Quantification of cell division in tailbud NMps. Quantification of increase in number of cells photolabelled using nls-kikume from (A) bud stage through to (B) 24 hpf. (C) Fold-change increase in labelled clone number changes depending on the labelled progenitor type, with regions contributing only to neural tissue undergoing most clonal expansion and bipotent progenitors undergoing the least clonal expansion. (D-F) Replicating cells stained using phospho-histone H3 (pH3) as a marker of mitotic cells (yellow) with bipotent NMps identified through co-expression of Sox2 (blue) and Ntl (red) at the (D) 22-somite stage, (E) 26-somite stage and (F) 32-somite stage. (G-I) The frequency of Ph3-positive nuclei in the (G) Ntl-positive expression domain, including notochord, (H) Sox2-positive expression domain, and (I) Ntl and Sox2-positive NMp domain. Two-tailed Student's t-test. **P<0.01, ****P<0.0001.