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Fig. S2
Zebrafish Vangl2 polyclonal antibody validation and Vangl2 overexpression. (A) Immunofluorescence labeling of endogenous Vangl2 protein expression in wild-type (WT) tailbud stage embryo (right panel, nuclei labeled with DAPI). (B) Vangl2 expression in a wild-type embryo injected with 200 pg synthetic vangl2 mRNA (right panel, nuclei labeled with DAPI). (C) Endogenous Vangl2 expression in a tailbud stage vangl2vu7/vu7 mutant embryo. Only background fluorescence from the secondary antibody is visible. Scale bars: 5 μm in panels A and B; 20 μm panel C.
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