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Fig. 5
Reactive oxygen species (ROS) in anaesthetized whole-mount Singapore wild-type zebrafish (Danio rerio) treated with equipotent concentrations (1 or 2× LC50) of Os-sulfonamide 1 or Os-azopyridine 10 for 96 h. Fluorescence for ROS (green) is shown superimposed onto bright field images. Embryos were stained using the green reagent of the ROS/Superoxide Detection Kit (Enzo life sciences) to detect ROS. Excitation, 458 and 488 nm; green emission for ROS, 493–550 nm. Rotenone was the positive control; 50 μM, 2 min exposure.(47)
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Full text @ J. Med. Chem.