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Fig. 1
Unsuccessful staining attempts. (A) Telencephalon of adult zebrafish stained with a protocol that reduced incubation times of the original BROPA protocol to a total duration of 2 weeks. Note severe tissue damage. (B) Telencephalon of adult zebrafish stained with a protocol that reduced incubation times of the original BROPA protocol to a total duration of 5 days without lead aspartate. Note charging artifacts in nuclei and neuropil.
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