ZFIN Image: Formella et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

Generation of mosaic KR expression utilising GAL4-UAS regulation. (A) The GAL4/upstream activating sequence (UAS) system is a powerful method for analysing cell function in vivo. The yeast GAL4 transcription factor activates the transcription of target genes by binding to UAS cis-regulatory sites. The Gal4/UAS system can be used as two-component gene expression system carried in separate lines. The driver line (Tg[met:Gal4; UAS:EGFP]) provides tissue-specific GAL4 expression and the responder line (Tg[4xnrUAS:MLS-KR, cryaa:EGFP]) carries the coding sequence for the gene of interest under the control of the UAS site. In the double transgenic F1 embryos, Gal4 expressing cells are visualized by fluorescent reporters, providing mosaic expression of the gene of interest (modified after Asakawa & Kawakami, 2008). (B) Zebrafish embryo at 3 dpf expressing both met:EGFP (green) and KR (red) (Tg[met:Gal4;UAS:EGFP;4xnrUAS:MLS-KillerRed, cryaa:EGFP]). (C-E) 5 dpf zebrafish larvae show mosaic expression for both KR-ve/EGFP+ve (C, E (green)), KR+ve/EGFP-ve (D, E (red)) and KR+ve/EGFP+ve (E (yellow)) motor neurons. All images are lateral views, anterior to the left, dorsal to the top. Scale bar 50 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.