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Fig. 5
Cancer modeling with TEAZ enables sequential electroporation of transgenes. A tumor-bearing fish (created with rb1 sgRNA as in Fig. 2) was imaged using GFP and tdTomato. As expected, only GFP+ tumor cells were seen, with no expression in the tdTomato channel. This tumor was then electroporated with an mitfa:tdTomato plasmid and re-imaged 5 days later, showing areas that are now both GFP+ and tdTomato+ (n=2).
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Full text @ Dis. Model. Mech.