ZFIN Image: Hu et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Gpc4, but not Vangl2, is required for convergence of the anterior endoderm.

(A-F) Expression of foxa2 (A-C) and sox17 (D-F) in the indicated embryos, as detected by WISH. Lateral (A-C, D-F) and anterior-dorsal (A-C) views. Yellow lines of equivalent length indicate width of the anterior endodermal sheets. Red lines of equivalent length indicate the distance between the lateralmost endodermal cells and the dorsal site of embryo. Red arrowheads indicate the end of anterior and posterior body axes. D, dorsal. (G,H) Epifluorescence images of anterior endoderm in control, vangl2 mutant Tg(sox17:EGFP) embryos at 4s. Anterior-dorsal view. White lines of equivalent length indicate width of the anterior endodermal sheets. A, anterior; P, posterior. (I) Quantification of endoderm width in each group of embryos shown in (G,H). Number of embryos for each group is indicated. Bars represent the mean±s.e.m. #, p>0.05; student’s t-test.

Acknowledgments

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