ZFIN Image: López-Muñoz et al., 2018, Fig. 8

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Figure Caption

Fig. 8

M. circinelloides promotes the recruitment and apoptosis of macrophages in the infection site. (A) Representative serial sections of zebrafish head kidneys challenged with PBS, 2.5×10⁵ R7B spores/fish and 10⁷ (high dose, HD) NRRL spores/fish at 16 hpi and immunostained with anti-Lcp1, anti-Mpx, and anti-active Casp3 antibodies. Infiltrated leukocytes (black arrows) at 16 hpi were PAS⁺, Lcp1⁺ and most of them Casp3⁺ in the fish infected with the virulent strain (R7B) and the HD of the avirulent NRRL strain. Scale bars 20 µm. (B) Quantification of Lcp1⁺, Mpx^+, and Casp3⁺ cells is shown as the mean ± SEM of the immunostained area/total area of 4 randomly distributed optical regions from 4 fish at ×200 magnification. ^*P<0.05.

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Phenotype details

Acknowledgments

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