ZFIN Image: Menon et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Heat shocks at end of pronuclear fusion perturbs the second centrosome duplication cycle in both 1C and 2C stalls. A–N: Immunofluorescence labelings of 18 mpfHS embryos for DNA (DAPI, blue), α‐tubulin (red), and γ‐tubulin (green). A–G: 1C stalls show loss of maternal PCM (A, B), PCM recovery and delayed entry into mitosis‐I (C), and mitosis‐I progression (D–G). H–N: 2C stalls show progression of mitosis‐I (H–K), monoasters in each cell of the two cells (L), subsequent centrosome duplication (M), and entry into mitosis‐II (N). In panels F, G, and K–N only one of the two cells is shown. Arrows indicate microtubule asters in the α‐tubulin panels and centrosomal foci in the γ‐tubulin panels. Each panel is a representative image of 9 to 17 embryos imaged for a particular time point.

Acknowledgments

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