ZFIN Image: Phan et al., 2018, Fig. S8

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Figure Caption

Fig. S8

NADPH oxidase inhibitor Apocynin does not affect muscle infection outcome.

(A-B-C) E. coli-DsRed were injected in the muscle of 2 dpf tg(mpx:GFP) embryos in DMSO or Apocynin treatment conditions. Bacteria (red) in the trunk region were imaged using fluorescent microscopy at 0 dpi and 1 dpi. (A) Representative bright field images overlaid with fluorescent channel of DMSO and Apocynin treated larvae. (B) Quantification of bacterial burden by Fluorescent Pixel Count (FPC) in indicated conditions (horizontal lines indicate the median values, N_DMSO = 18–19 and N_APO = 16–17, Kruskal-Wallis’ test with Dunn’s post-test, *** p<0.001). Larva diagram shows the region of counting. (C) Survival curves of DMSO and Apocynin treated larvae infected with E. coli in the muscle from 0 to 3 dpi or injected with PBS (N_larvae is indicated in the figure, log rank test, p>0.05, ns: not significant).

Acknowledgments

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