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Fig. 4
Overexpression of C. semilaevis Rspo3 affects Danio rerio embryo development. An amount of 1000 pg/embryo of C. semilaevis Rspo3 mRNA was injected into AB strain D. rerio embryos at 1–4 cell stages. All embryos are shown with anterior to the left. Scale bar = 200 μm. (A) Classification of phenotypes at 24 h post-fertilization (hpf); (B) Classification of phenotypes at 54 hpf. The red arrowheads indicate ecchymosis; (C) The percentages of embryos in each category as shown in (A) and (B); N is the number of total samples analyzed in each group.
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Full text @ Int. J. Mol. Sci.