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Fig. S5
Histology of testis from wildtype (ar +/+), and homozygous (ar -/-) (the mutant 1) zebrafish at 1.5 mpf, 70dpf and 5 mpf. (A, B) Histology of testes from the wildtype (ar +/+) and homozygous (ar -/-) (ar ihb1225/ihb1225) zebrafish at 1.5 mpf. No obvious difference between ar -/- and wildtype sibling (ar +/+) testes. SGC, spermatogonial cysts (indicated with red arrows). (C, D) Histology of testes from the wildtype (ar +/+) and homozygous (ar -/-) (ar ihb1225/ihb1225) zebrafish at 70 dpf. Compared with wildtype sibling testes, spermatogenesis of ar-/- testes was delayed as indicated by more spermatogonia (SG) and fewer spermatocytes (SC), but no spermatozoa (SZ). (E, F) Histology of testes from the wildtype (ar +/+) and homozygous (ar -/-) zebrafish at 6 mpf. ar -/- testes were degenerated and loose; but spermatozoa were filled in wildtype sibling (ar +/+) testes. Dpf, days post fertilization; Mpf, months post fertilization.