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Fig. 6

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ZDB-IMAGE-180808-4
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Figures for Yu et al., 2018
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Fig. 6

Targeted disruption of ar in zebrafish causes premature ovarian failure during growth. (A-C) Gross appearance and ovaries from wildtype (ar +/+), heterozygous (ar +/-) and homozygous (ar -/-) (ar ihb1225/ihb1225) female zebrafish. At 4.5 mpf, the gross appearance of ar -/- (ar ihb1225/ihb1225) female zebrafish was indistinguishable from wildtype siblings (ar +/+) or their heterozygous sibling (ar +/-), but the ovaries of ar -/- female were smaller with fewer eggs and became transparent compared with wildtype sibling (ar +/+) or heterozygous siblings (ar +/-). (D) Gonadosomatic index (GSI) in ar +/+ and ar -/- female zebrafish. (E) Fertilization rate of eggs from wildtype (ar +/+) and homozygous (ar -/-) female zebrafish at 4mpf. (F, G) Histology of ovaries from wildtype (ar +/+) and homozygous (ar -/-) (ar ihb1225/ihb1225) zebrafish at 1.5 mpf. No obvious difference between ar -/- and ar +/+ ovaries. OC, Oocyte. (H, I) Histology of ovary from the wildtype (ar +/+) and homozygous (ar -/-) (ar ihb1225/ihb1225) zebrafish at 70 dpf. Ovaries of wildtype (ar +/+), most oocytes developed to stage III and IV; but more ovaries of homozygous (ar -/-) (M1) developed to stage II or I, some developed to stage III and IV. (J, K) Histology of ovaries from wildtype (ar +/+) and homozygous (ar -/-) zebrafish at 4 mpf. In ovaries of wildtype (ar +/+), most oocytes developed to stage IV, but for ovaries of homozygous (ar -/-), most oocytes developed to stage II and partial degeneration occurred. (L, M) Histology of ovaries from wildtype (ar+/+) and homozygous (ar -/-) zebrafish at 4 mpf. In ovaries of homozygous (ar -/-), follicles atresia and degeneration occurred. (N, O) Histology of ovaries from wildtype (ar +/+) and homozygous (ar -/-) zebrafish at 5 mpf. In ovaries of homozygous (ar -/-), no follicles were observed and severe degeneration occurred (30%, n = 30). (P) lhcgr (luteinizing homone/choriogonadotropin receptor) expression in ovaries of wildtype (ar+/+) and homozygous (ar-/-) zebrafish at 4.5 mpf. (Q) foxl2 (forkhead box L2) expression in ovaries of wildtype (ar +/+) and homozygous (ar -/-) zebrafish at 4.5 mpf. (R) cyp11a1 (p450 side chain cleavage enzyme) expression in ovaries of wildtype (ar+/+) and homozygous (ar -/-) zebrafish at 4.5 mpf. (S) kitlga expression in ovaries of wildtype (ar+/+) and homozygous (ar -/-) zebrafish at 4.5 mpf injected with or without DHT (100 nM, 10μl for each). Dpf, days post fertilization; Mpf, months post fertilization.

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