ZFIN Image: Takayama et al., 2018, Fig. S4

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Figure Caption

Fig. S4

Detection of apoptotic cells by NH₄Cl- or CQ-treatment.

(A) Experimental scheme. NH₄Cl or chloroquine (CQ) was treated from -12 to 3 or 24 hpa. (B-G) Longitudinal ray sections and quantification of TUNEL positive cells/total cell number per area that consists of the whole regenerates and 500 µm below the amputation plane in control, NH4Cl-, or CQ-treated fin stumps at 3 and 24 hpa; apoptotic cells and nuclei were visualized by immunohistochemical staining and DAPI staining, respectively (n = 5). Representative images (B-G) used for quantification are shown in H. White dashed lines indicate the amputation planes. Scale bars: 50µm (B-G). n.s.: not significant. Error bars represent the standard error.

Acknowledgments

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