ZFIN Image: Schredelseker et al., 2018, Fig. S4

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Figure Caption

Fig. S4

bsx expression in embryos with altered Nodal signaling.

(A-D) bsx expression analyzed by whole mount in situ hybridization, dorsal views, anterior at top; scale bar: 50 µm. (A) bsx is expressed normally (see Fig. 1D) in the pineal gland in GFP encoding mRNA injected wildtype control embryos at 30 hpf (n=18/18). (B) In embryos injected with lefty mRNA, bsx is expressed in two bilateral domains in the putative pineal anlage at 30 hpf in embryos (n=23/26), which may be caused by the fact that the neural tube fails to close in lefty overexpressing embryos. (C) At 2 dpf bsx is expressed asymmetrically in the parapineal on the left side of the pineal complex of wildtype siblings or ntl^b160 heterozygous embryos (phenotypically considered wildtype siblings). (D) in contrast, in ntl^b160 homozygous siblings, sidedness of parapineal bsx expression appears to be randomized, as the bsx domain was found in n=5/12 embryos on the right side of the pineal (parapineal cells were displaced to the left in 6/12 embryos; 1/12 embryos showed bsx expression only in the pineal gland and not displaced from the midline).

Acknowledgments

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