ZFIN Image: Fassier et al., 2018, Fig. S3

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Figure Caption

Fig. S3

Fignl1 depletion does not perturb the specification, localization, or survival of SMNs. (A) Immunolabeling of SMNs (MNs), RB sensory neurons, and GABAergic interneurons in 26-hpf MO^Ctl and MO^Fignl1 embryos with Islet1/2 (green) and GABA (red) antibodies. Lateral views of the trunk; anterior is to the left. Bar, 50 μm. (B and C) Mean number (Nb.) of MNs, RB neurons, and GABAergic interneurons (GABA). Quantifications were performed on 26-hpf control (n = 22) and morphant (n = 21) embryos pooled from three independent experiments. 12 hemisegments were analyzed in the anterior (B) and posterior regions (C) of each embryonic trunk. ***, P ≤ 0.001; unpaired two-tailed t test. Error bars are SEM. (D) Immunolabeling of activated caspase 3 (red) and the neuronal postmitotic marker HuC/D (green; left) or caspase 3 alone (red; right) in 26- (left) or 56-hpf (right) MO^Ctl and MO^Fignl1 embryos and larvae. Insets represent higher magnifications of the indicated boxed regions. Fignl1 knockdown does not induce apoptotic cell death of spinal neurons. Bars, 30 μm.

Acknowledgments

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