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Fig. 6
Reactive oxygen species (ROS) production by 2,7-dichlorodihydrofluorescein diacetate (DCHF-DA) assay. For evaluation of ROS generation, wild type zebrafish was used with DCHF-DA assay on hair cells as in green dots in the middle row. A 4´,6-diamidino-2-phenylindole (DAPI)-positive cells were observed as blue fluorescent dots as in the lower row. By merging those figures, the ROS production cells could be clearly identified as in the upper row. Neomycin as positive control showed strongest ROS generation. Despite not strong as in neomycin, there was increased ROS generation in hair cells in nicotine 40 μM. O1, otic.
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Full text @ Clin. Exp. Otorhinolaryngol.