Fig. 2

Heart morphology evaluation and changes in heart rate induced by nicotine treatment. (A) Merged images from the fluorescent image and optical image are shown. There was no difference in heart size between groups (green area). All images were captured using transgenic (cmlc2:EGFP) zebrafish at 72 hours post-fertilization (hpf, ×16). Scale bar=200 μm. (B) The heart rate of zebrafish embryos was significantly decreased in nicotine-exposed groups (P <0.001, one-way analysis of variance). All data were evaluated at 72 hpf. Only statistically significant pair-wise comparisons in post-hoc analysis are shown. *Statistically B significant (P<0.05, total n=150).