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Fig. 9
Projections of 120A-Dm neurons. a Dorsal view of the brain. The positions of the sections are shown as bars (b–g). b–d Immunofluorescence staining using anti-GFP. b, c Coronal sections of SAGFF70A;UAS:GFP;UAS:zBoTxBLC:GFP fish. d Sagittal section of SAGFF120A;UAS:GFP;UAS:zBoTxBLC:GFP fish. Axons of the 120A-Dm neurons project to the hypothalamus area. Arrowheads in b and c indicate projections from two groups of the 120A-Dm neurons. Arrows in c and d indicate the lateral forebrain bundle. e–g Double immunofluorescence staining using anti-GFP (green) and anti-MAP2 (magenta; dendritic marker) of the brain of SAGFF120A;UAS:GFP fish. Schemes of coronal views of the telencephalon are shown on the left. e Projections to Vd. f Projections to Vs. e, f Arrowheads mark projections that were not co-stained with anti-MAP2. g Projections to the neuropil area of EN and Ppa. Cell bodies in EN and Ppa were stained with DAPI (blue). Dm, medial zone of dorsal telencephalic area (D); Dl, lateral zone of D; Dc, central zone of D; Dp, posterior zone of D; SY, sulcus ypsiloniformis; Vd, dorsal nucleus of ventral telencephalic area (V); Vv, ventral nucleus of V; EN, entopeduncular nucleus; Ppa, Parvocellular preoptic area; Cand, Commissura anterior, pars dorsalis; Cantv, Commissura anterior, pars ventralis. h A schematic view of axonal projections of the 120A-Dm neurons (green) to Vd, Vs, EN, Ppa, and the hypothalamus. i Light-sheet microscopy of a cleared brain from the SAGFF120A;UAS:GFP fish. Horizontal section (left) and coronal sections (right) showed projections of the 120A-Dm neurons terminated in the lateral hypothalamic nucleus (LH), the anterior tuberal nucleus (ATN), and dorsal zone of periventricular hypothalamus (Hd). Scale bars, 200 μm (b–d), 50 μm (e, g), 25 μm (f), and 500 μm (i)