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Fig. S3
Kidney marrow-derived cells do not contribute to collagen deposition during fibrotic response to cryoinjury. (A) Kidney marrow-derived cells from a transgenic line ubiquitously expressing GFP and mCherry under the control of col1a2 regulatory regions were transplanted into irradiated wild-type adult zebrafish. After kidney marrow reconstitution, host hearts were cryoinjured. Expression of mCherry and GFP was assessed at 7 dpi by immunofluorescence of sections. MHC marks the myocardium, and nuclei are counterstained with DAPI. (B) Whole-heart section of a transplanted heart at 7 dpi. (C–E) Merged and single channels of the boxed area in B. No mCherry signal is visible. (F) Control whole-heart section from a col1a2:mCherry transgenic fish at 7 dpi. (G–I) Merged and single channels of the boxed section shown in F; mCherry signal is detected in the epicardium and injury areas. Asterisks indicate the injury area. [Scale bars, 25 μm (C and G) and 100 μm (B and F).]