ZFIN Image: Sánchez-Iranzo et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Genetic ablation of collagen 1a2-expressing cells impairs cardiomyocyte proliferation in the cryoinjured heart. (A) col1a2:mCherry-NTR adult animals were cryoinjured and treated with metronidazole (Mtz) from 4 to 6 dpi. BrdU injection was performed 1 d before fixation. (B–G) Immunofluorescence using anti-mef2 and anti-MHC to mark cardiomyocytes, and anti-BrdU in col1a2:loxP-tagBFP-loxP-mCherry-NTR (control) and col1a2:mCherry-NTR fish treated with Mtz and BrdU as described in A. Arrowheads mark BrdU⁺ cardiomyocytes. (H) Quantification of BrdU⁺ cardiomyocytes in col1a2:mCherry-NTR and control hearts. Shown are individual measurements and median ± interquartile range; ***P = 0.0004 by Mann–Whitney test, n = 23 fish per condition, from two different experiments (blue and red dots). Three whole-heart ventricle sections were quantified per point. [Scale bars, 10 µm (C and E–G) and 100 µm (B and D).]

Acknowledgments

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