IMAGE

Fig. 3

ID
ZDB-IMAGE-180711-63
Source
Figures for Kontarakis et al., 2018
Image
Figure Caption

Fig. 3

flt4+/- larvae are sensitive to mild miR-126 knockdown. (A) Design of the experiment: embryos from a flt4+/- outcross were injected with a low dose of mir-126 MO, scored for lymphatic defects at 5 dpf, and then genotyped. (B, C) Facial lymphatic analysis of flt4+/- and wild-type siblings injected with 1 ng of mir-126 MO. Uninjected larvae of both genotypes develop a functional facial lymphatic network. However, unlike their wild-type siblings, flt4+/- larvae display facial lymphatic defects (asterisk) when injected with 1 ng of mir-126 MO. Graph shows data from 3 independent experiments. Total number of larvae analysed: 73 (35 flt4+/- and 38 flt4+/+). (D,E) Thoracic duct (TD) analysis. Representative side views for normal, partial, and no TD development; asterisks indicate lack of TD formation; two suboptimal doses of mir-126 MO (0,5 ng and 1 ng) were used. Wild-type siblings display a largely complete TD; flt4+/- injected with mir-126 MO resemble mir-126 mutants. Results from 4 independent experiments; 16 larvae analysed per experiment. Unpaired Student's t-test was performed to test for statistical significance.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.