ZFIN Image: Trivedi et al., 2018, Fig. 4

Image

Figure Caption

Fig. 4

Measuring a twofold difference in mRNA levels. (A) Two-channel imaging of citrine (red channel, Alexa 647) and desma (green channel, Alexa 546) target mRNAs in homozygous Gt(desma-citrine)^{ct122a/ct122a} embryos and heterozygous Gt(desma-citrine)^ct122a/+ embryos. Confocal microscopy: 0.7×0.7 µm pixels. Whole-mount zebrafish embryos fixed at 26 hpf. Depicted regions are analyzed in B-D. (B) Normalized signal for citrine (red) and desma (green) targets in homozygous and heterozygous embryos (mean±s.d. via uncertainty propagation, n=3 embryos). (C) Ratio of citrine target in homozygous versus heterozygous embryos (mean±s.d. via uncertainty propagation, n=3 embryos). (D) Normalized signal for 2×2×2 µm voxels within the selected regions of A. See section S2.4 in the supplementary material for additional data.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development