Fig. 5

Combinatorial activation of Shh signaling and inhibition of adamts5 rescues paraxial myod expression. (A) Adaxial and paraxial myod expression in 12 hpf embryos treated with vehicle control (a–c, arrows), 10 μM Smoothened agonist (Smo agon.) (d–f, arrow/open arrowhead represent present/absent myod expression in paraxial mesoderm), vehicle + adamts5-MO (g–i, arrow/open arrowhead represents present/absent myod expression in paraxial mesoderm) and 10 μM Smo agon. + adamts5-MO (j–l, arrows indicate myod expression present in paraxial mesoderm). Scale bar = 100 μm; (B) Quantitation of embryos showing present or absent myod patterning represented in (A).