Fig. 2

Notochord morphogenesis and muscle fiber formation is perturbed in adamts5 morphant embryos. (A) Expression of shh in the notochord of 18 hpf control (a,a′, arrows) and adamts5 morphant (b,b′, open arrowheads) embryos, with medio-lateral deviation in the adamts5 morphants (b,b′), with anterior indicated (*); (B) Expression of ntl in the notochord of 18 hpf control (a,a′, arrows) and adamts5 morphant (b,b′, open arrowheads) embryos, with medio-lateral deviation in the adamts5 morphants with anterior indicated (*); (C) Expression of myod in adaxial and paraxial mesoderm of 18 hpf control embryos (a,a′) and its perturbation in adamts5 morphants (b,b′, open arrowheads) with anterior indicated (*); (D) Quantitation of affected notochords in control and adamts5 morphant embryos demarcated by shh in Figure 2A; (E) Quantitation of affected notochords in control and adamts5 morphant embryos demarcated by ntl in Figure 2B; (F) Quantitation of embryos with perturbed myod expression in control and adamts5 morphant embryos demarcated in Figure 2C; (G) Double-transgenic Tg(acta1:lifeact-GFP)/Tg(acta1:mCherryCaaX) embryos, in which thin filaments are marked green and sarcolemma red, reveal loss of muscle integrity in 3 dpf adamts5 morphants. Muscle fibers of control injected larvae feature the typical striation of the myofibril and regular myofibers within chevron-shape somites, indicated by a dashed line (a). The boxed area in a is magnified in a′–a′′′. Myofibril striation is partially lost within adamts5 morphants (arrowhead in b′) and the sarcolemma of the myofibers disrupted (arrow in b′′). The boxed area in b is magnified in b′–b′′′. Scale bar = 50 μm.