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Fig. 4
MTZ-treated embryos develop into male-like adult zebrafish with shrunken testis 100-dpf adult zebrafish developed from control or MTZ-treated embryos. Both male and female zebrafish were found in the control (A), while all the adult zebrafish derived from the embryos treated with MTZ (B) were male-like with typical sexually pigmentation. The female/male ratio in control (C) or MTZ treatment group (D) were also analyzed. Dissected the testis from adult control and MTZ-treated zebrafish revealed that the testis of treated fish were shrunken, significantly smaller than those of controls (E,F).
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