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Fig. 2
Four days old Gateways larvae express GFP in the OVLT. Confocal microscopy of compound transgenics Gateways (GFP, green)/Tg (kdrl:ras-Cherry) (red) in vivo reveals the OVLT in respect of developing vasculature. (A) Frontal view at the level immediately anterior to the optic chiasm; (B) dorsal view at the level of OVLT; (B') schema of the OVLT in respect of vasculature (based on the vasculature atlas, Isogai et al., 2001). cmv, communicating vessel; crdi, cranial division of the internal carotid artery; d, diencephalon; ht, hypothalamus; e, eye; mplv, median palatocerebral vein; oc, optic chiasm; ol, olfactory placode; on, optic nerve; ovlt, organum vasculosum laminae terminalis; pla, palatocerebral artery. Scale bar−50 μm.