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Fig. 1
Transgenic zebrafish (Gateways) expresses GFP in several CVOs and migratory microglia. (A–F) whole mounts (anterior to the left); (A,B) in vivo; (C,D) anti-GFP WISH (eyes and olfactory placode removed); (A–E) lateral view; (F) frontal view; (E,F) double immunohistochemistry (anti-GFP–green, anti-acetyl-tubulin–red; (E) eyes and olfactory placode removed), (G,H) anti-GFP immunohistochemistry on cross-sections. Scheme indicates the level of cross-sections shown in (G,H). ac, anterior commissure; ah, adenohypophysis; c, cerebellum; cpIII, choroid plexus of III ventricle; cpIV, choroid plexus of IV ventricle; d, diencephalon; e, epiphysis; h, hour postfertilization; hb, hindbrain; ht, hypothalamus; mhb, midbrain-hindbrain boundary; me, median eminence; mg, migratory microglia; oc, optic chiasm; ol, olfactory placode; on, optic nerve; ot, optic tectum; ovlt, organum vasculosum laminae terminalis; poc, postoptic commissure; t, telencephalon; tc, tela choroidea; #, subfornical organ. Scale bar−50 μm.