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Fig. S6
Characterisation of epithelial integrity marker and grhl-target gene E-cadherin following modulation of grhl3 and arhgef19 activity at 24 hpf. (A-C) The expression of E-cadherin (A) and actin (B) in the EVL (C; merged) of WT fish overlying the region of the midbrain-hindbrain boundary (MHB) is unchanged following MO-mediated knockdown of grhl3 (D-F), arhgef19 (G-I), both grhl3 and arhgef19 together (J-L), or over-expression of grhl3 mRNA (M-O) at 24 hpf. Note increased EVL cell size in both MO:grhl3 (D-F) and MO:grhl3+MO:arhgef19 (J-L) injected embryos. Nuclei in merged panels (C, F, I, L, O) are shown by DAPI staining.