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Fig. 6
We used focal plane scanning OPT (FPS-OPT) to image the head of a Tg(fli1a:eGFP) zebrafish in 3D fluorescence with a 10 ×/0.3 NA air objective. Under such conditions, single-plane OPT would be unable to produce an acceptable reconstruction due to the large sample thickness and shallow depth-of-field. With FPS-OPT, we compare 3D reconstructions from (a) standard filtered backprojection (FBP) and (b) our proposed PSF-aware FBP. Our proposed PSF-aware FBP algorithm reconstructs an image with less out-of-focus blur. Scalebar is 100 μm.
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Full text @ Biomed. Opt. Express