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Fig. 1 Evaluation of mineralization in alendronate-treated zebrafish. (A) The gross morphology of zebrafish aged at 7 dpf which have been treated with different concentrations of alendronate (10, 20, and 30 µM, left bright-filed panel) from 3 dpf onwards. Calcein staining on control and alendronate-treated embryos at 7 dpf (right green fluorescent panel); (B) Quantification of mineralization degree by detecting the fluorescence intensity at the area of centrum form ring in the notochord. (values are mean ± SD; tested by one-way ANOVA pairwise comparison; N = fish number; Different letters indicate significant differences) (C) Chemical structure of alendronate.