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Fig. 4

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ZDB-IMAGE-180417-39
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Figures for Shi et al., 2017
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Fig. 4

Rbpr2 musc97 mutants show eye and systemic phenotypes consistent with retinoid deficiency. Lateral view of representative WT (panels A,C) and rbpr2 homozygous mutant (rbpr2 musc97 mut, panels C,D) zebrafish at 5.5 dpf. Rbpr2 musc97 mutants showed gross defects, which included: *hydrocephaly; **smaller eyes, ***pericardial edema and ****slight tail curvature. Scale bars = 0.326 mm (A,B) and 0.103 mm (C,D). (E) qPCR analysis of rbpr2 mRNA expression from WT and rbpr2 musc97 mutant zebrafish larvae at 5.5 dpf. (F) Injection of WT zebrafish rbpr2 mRNA or dose specific treatment with all-trans retinoic acid (ATRA) rescues the rbpr2 musc97 mutant phenotype. Images obtained at 5 dpf. Rescue experiments of rbpr2 musc97 mutants with either mRNA or ATRA were repeated twice as outlined in methods. (G,H) Transverse sections of 5.5 dpf WT (panel G) and rbpr2 musc97 mutant (panel H) eyes. rbpr2 musc97 homozygous mutant eyes were smaller and show disruption of retinal lamination layers.

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