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Fig. 2
Enzalutamide inhibits testosterone-induced proliferation of xenografted LNCaP cells in vivo. (A) Representative brightfield and fluorescent images of casper embryos injected with CMTMR labeled LNCaP cells at 24 hpi (baseline) and 96 hpi (endpoint) without and with the addition of enzalutamide (5 µM) alone or with testosterone (125 nM). Scale bar = 200 microns. (B) Quantification of fluorescently labeled XT LNCaP cells showed that the addition of 5 µM of enzalutamide (ENZ) significantly decreased cell proliferation caused by the addition of 125 nM of testosterone (T) in the zebrafish model. Error Bars = Mean ± SEM (N = 4); *P < 0.05, **P < 0.01 for significant differences in cell numbers determined using the Student’s t-test. Groups of 20 embryos were sacrificed per replicate.