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Fig. 3 (A) Images of 120 hpf ERE-TG zebrafish larvae exposed to 1.0 mg/L BPA in the presence and absence of estrogen inhibitor ICI 182 780, scale bar represents 200 μM. (B–D) GFP induction (fluorescence) in 120 hpf ERE-TG zebrafish larvae exposed to bisphenol chemicals in combination with estrogen inhibitor ICI 182 780 measured as fold increase in GFP above control in the (B) heart, (C) liver, and (D) tail somites. Bisphenol concentrations were 1.0 mg/L for BPA and BPF, 0.10 mg/L for BPAF, and 20 mg/L for BPS, when included ICI 182 780 concentration was 607 μg/L. Data are reported as mean ± SEM (asterisk indicate significant difference compared with the control, *p < 0.05 and ** p < 0.01).