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Fig. s15 Effects of various OTG deletion mutants in zebrafish development
(A-F) Morphology (at 36 hpf) of control embryo (A, 12/12, 100%) and embryos injected with mRNA for indicated OTG deletion mutant. Overexpression of OTGPBD-Flag results enlarged head and shortened tail (F, 15/16, 94%) similar to wild type OTG (B, 15/15, 100%). OTG TM-Flag (C, 10/14, 71%) and OTGPRD-Flag (E, 12/16, 75%) lead to small head with normal tail. OTGTM-Flag does not cause any discernible defect. (G-L) Immunostaining of overexpressed Fz8-Myc (green) in shield stage embryos injected with mRNA for fz8-myc alone (G) or with mRNA for indicated OTG-Flag deletion mutant. Overexpressed Fz8-myc proteins (green) are visualized by anti-Myc immunostaining. Nuclei are counter-stained with Hoechst 33342 (blue). Cell surface expression of Fz8-Myc is affected by OTG-Flag (H) and OTGPBD-Flag (L). However, OTG TM-Flag (I), OTGTM- Flag (J), and OTGPRD-Flag (K) do not affect the membrane localization of Fz8-Myc.